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blue white selection lb agar plates  (Teknova)


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    Structured Review

    Teknova blue white selection lb agar plates
    Blue White Selection Lb Agar Plates, supplied by Teknova, used in various techniques. Bioz Stars score: 96/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blue+white+selection+lb+agar+plates/LB+Agar+Plates/pmc12677897-231-26-31
    Average 96 stars, based on 150 article reviews
    blue white selection lb agar plates - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Construct:

    Article Title: Mechanism of SK2 channel gating and its modulation by the bee toxin apamin and small molecules
    Article Snippet: The amino acid sequences for SK2-4-GFP and human calmodulin (CaM) (CALM1, Uniprot P0DP23, 149 amino acids) were codon optimized using Twist Bioscience’s tool to create DNA sequences for expression. .. Plasmids encoding the constructs (SK2-4-GFP, CaM) were each separately transformed into E. coli DH10Bac competent cells (ThermoFisher Scientific) following the manufacturer’s protocol & plated onto blue/white selection LB agar plates (Teknova). .. The pWIL-BacMam vector contains the Tn7 transposon and encodes a baculovirus genome with a multiple cloning site, such that when a gene of interest is placed in the pWIL-BacMam vector and transformed into E. coli DH10Bac competent cells (ThermoFisher Scientific) it produces bacmid containing the gene of interest.

    Article Title: Mechanism of SK2 channel gating and its modulation by the bee toxin apamin and small molecules
    Article Snippet: .. Plasmids encoding the constructs (SK2–4-GFP, CaM) were each separately transformed into E. coli DH10Bac competent cells (Thermo Fisher Scientific) following the manufacturer’s protocol and plated onto blue/white selection LB agar plates (Teknova). .. After 2 days of incubation at 37°C, a white colony for each construct was selected and grown overnight in 5 ml liquid medium containing 7 μg/ml gentamicin sulfate, 50 μg/ml kanamycin, and 10 μg/ml tetracycline hydrochloride (Teknova).

    Transformation Assay:

    Article Title: Mechanism of SK2 channel gating and its modulation by the bee toxin apamin and small molecules
    Article Snippet: The amino acid sequences for SK2-4-GFP and human calmodulin (CaM) (CALM1, Uniprot P0DP23, 149 amino acids) were codon optimized using Twist Bioscience’s tool to create DNA sequences for expression. .. Plasmids encoding the constructs (SK2-4-GFP, CaM) were each separately transformed into E. coli DH10Bac competent cells (ThermoFisher Scientific) following the manufacturer’s protocol & plated onto blue/white selection LB agar plates (Teknova). .. The pWIL-BacMam vector contains the Tn7 transposon and encodes a baculovirus genome with a multiple cloning site, such that when a gene of interest is placed in the pWIL-BacMam vector and transformed into E. coli DH10Bac competent cells (ThermoFisher Scientific) it produces bacmid containing the gene of interest.

    Article Title: Mechanism of SK2 channel gating and its modulation by the bee toxin apamin and small molecules
    Article Snippet: .. Plasmids encoding the constructs (SK2–4-GFP, CaM) were each separately transformed into E. coli DH10Bac competent cells (Thermo Fisher Scientific) following the manufacturer’s protocol and plated onto blue/white selection LB agar plates (Teknova). .. After 2 days of incubation at 37°C, a white colony for each construct was selected and grown overnight in 5 ml liquid medium containing 7 μg/ml gentamicin sulfate, 50 μg/ml kanamycin, and 10 μg/ml tetracycline hydrochloride (Teknova).

    Selection:

    Article Title: Mechanism of SK2 channel gating and its modulation by the bee toxin apamin and small molecules
    Article Snippet: The amino acid sequences for SK2-4-GFP and human calmodulin (CaM) (CALM1, Uniprot P0DP23, 149 amino acids) were codon optimized using Twist Bioscience’s tool to create DNA sequences for expression. .. Plasmids encoding the constructs (SK2-4-GFP, CaM) were each separately transformed into E. coli DH10Bac competent cells (ThermoFisher Scientific) following the manufacturer’s protocol & plated onto blue/white selection LB agar plates (Teknova). .. The pWIL-BacMam vector contains the Tn7 transposon and encodes a baculovirus genome with a multiple cloning site, such that when a gene of interest is placed in the pWIL-BacMam vector and transformed into E. coli DH10Bac competent cells (ThermoFisher Scientific) it produces bacmid containing the gene of interest.

    Article Title: Mechanism of SK2 channel gating and its modulation by the bee toxin apamin and small molecules
    Article Snippet: .. Plasmids encoding the constructs (SK2–4-GFP, CaM) were each separately transformed into E. coli DH10Bac competent cells (Thermo Fisher Scientific) following the manufacturer’s protocol and plated onto blue/white selection LB agar plates (Teknova). .. After 2 days of incubation at 37°C, a white colony for each construct was selected and grown overnight in 5 ml liquid medium containing 7 μg/ml gentamicin sulfate, 50 μg/ml kanamycin, and 10 μg/ml tetracycline hydrochloride (Teknova).



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    96
    Teknova blue white selection lb agar plates
    Blue White Selection Lb Agar Plates, supplied by Teknova, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blue+white+selection+lb+agar+plates/LB+Agar+Plates/pmc12677897-231-26-31
    Average 96 stars, based on 1 article reviews
    blue white selection lb agar plates - by Bioz Stars, 2026-09
    96/100 stars
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